Reproducible propagation technique for the symbiotic cnidarian model system Cassiopea xamachana
Author:
Newkirk Casandra1ORCID,
Vadlapudi Sankalp1,
Sadula Mahita1,
Arbello Cheri1,
Xiang Tingting1
Affiliation:
1. The University of North Carolina at Charlotte Department of Biological Sciences , , Charlotte, NC 28223 , USA
Abstract
ABSTRACT
The phylum Cnidaria is composed of corals, jellyfish, hydras, and sea anemones. Cnidarians are well-known for their regenerative capability, with many species maintaining the ability to regenerate complete structures. This regenerative capacity has been used casually for propagation purposes (via dissection) for some cnidarians used in laboratory research but has yet been documented in a manner meant to be reproducible. One such cnidarian model system is the scyphozoan jellyfish Cassiopea xamachana. C. xamachana has become an emerging model system for studying the cnidarian-algal symbiotic relationship, so determining a reliable and fast method for expansion of laboratory animals is crucial. Here we outline a reproducible propagation method for continued generation and growth of C. xamachana polyps.
This article has an associated First Person interview with the first author of the paper.
Funder
University of North Carolina
University of California, Riverside
National Science Foundation
NC Biotechnology Flash Grant Award
Publisher
The Company of Biologists
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology
Cited by
1 articles.
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