Central ventilatory and cardiovascular actions of calcitonin gene-related peptide in unanesthetized trout

Abstract

SUMMARYCalcitonin gene-related peptide (CGRP) and its receptors are widely distributed in the tissues of teleost fish, including the brain, but little is known about the ventilatory and cardiovascular effects of the peptide in these vertebrates. The present study was undertaken to compare the central and peripheral actions of graded doses (5–50 pmol) of trout CGRP on ventilatory and cardiovascular variables in unanesthetized rainbow trout. Compared with vehicle, intracerebroventricular injection of CGRP significantly elevated the ventilation frequency (fV) and the ventilation amplitude (VAMP) and, consequently, the total ventilation (VTOT). The maximum hyperventilatory effect of CGRP (VTOT: +300%), observed at a dose of 50 pmol, was mostly due to its stimulatory action on VAMP (+200%) rather than fV (+30%). In addition, CGRP produced a significant and dose-dependent increase in mean dorsal aortic blood pressure (PDA) (50 pmol: +40%) but the increase in heart rate (fH) was not significant. Intra-arterial injections of CGRP were without effect on the ventilatory variables but significantly and dose-dependently elevated PDA (50 pmol: +36%) without changing fH. At the highest dose tested, this hypertensive phase was preceded by a rapid and transient hypotensive response. In conclusion, our study suggests that endogenous CGRP within the brain of the trout may act as a potent neurotransmitter and/or neuromodulator in the regulation of cardio-ventilatory functions. In the periphery, endogenous CGRP may act as a local and/or circulating hormone preferentially involved in vasoregulatory mechanisms.