Observation of cultured embryonic epithelial cells in side view

Abstract

Epithelial cells from the early chick embryo were explanted and grown on a strip of coverglass which was positioned in a culture chamber in such a way that the cells could be filmed in side view. Ruffling of the cells at the leading edge of the sheet (marginal cells) appeared similar to the ruffles at the leading lamella of fibroblasts. The ruffles of the sub-marginal cells showed different characteristics, being longer and not propagated successively as were the marginal ruffles. Superimposition of cells was always the result of the active movement of a non-yolky cytoplasmic process underneath a yolky process (i.e. underlapping). The non-yolky processes appeared to be strongly adherent to the substrate in comparison with the yolky processes, which were easily displaced. Active movement of cells or lamellae on the dorsal surface of the sheet was never observed. Preliminary ultrastructural evidence is presented which suggests that the strongly adherent non-yolky processes possess well-defined microfilament tracts associated with the ventral plasma membrane, while the poorly adherent processes do not possess such tracts.