Deletion of the Ebf1, a mouse deafness gene, causes a dramatic increase in hair cells and support cells of the organ of Corti

Author:

Powers Kathryn G.12,Wilkerson Brent A.3ORCID,Beach Kylie E.1,Seo Sophie S.1,Rodriguez Jose S.1,Baxter Ashton N.3,Hunter Sarah E.3,Bermingham-McDonogh Olivia1ORCID

Affiliation:

1. University of Washington School of Medicine 1 Department of Biological Structure , , Seattle, WA 98195 , USA

2. University of Washington 2 Program in Molecular and Cellular Biology , , Seattle, WA 98195 , USA

3. Medical University of South Carolina 3 Department of Otolaryngology-Head and Neck Surgery , , Charleston, SC 29425 , USA

Abstract

ABSTRACT Following up on our previous observation that early B cell factor (EBF) sites are enriched in open chromatin of the developing sensory epithelium of the mouse cochlea, we investigated the effect of deletion of Ebf1 on inner ear development. We used a Cre driver to delete Ebf1 at the otocyst stage before development of the cochlea. We examined the cochlea at postnatal day (P) 1 and found that the sensory epithelium had doubled in size but the length of the cochlear duct was unaffected. We also found that deletion of Ebf1 led to ectopic sensory patches in the Kölliker's organ. Innervation of the developing organ of Corti was disrupted with no obvious spiral bundles. The ectopic patches were also innervated. All the extra hair cells (HCs) within the sensory epithelium and Kölliker's organ contained mechanoelectrical transduction channels, as indicated by rapid uptake of FM1-43. The excessive numbers of HCs were still present in the adult Ebf1 conditional knockout (cKO) animal. The animals had significantly elevated auditory brainstem response thresholds, suggesting that this gene is essential for hearing development.

Funder

National Institute on Deafness and Other Communication Disorders

National Institute of General Medical Sciences

Hollings Cancer Center

Virginia Merrill Bloedel Hearing Research Center

University of Washington

Medical University of South Carolina

Publisher

The Company of Biologists

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