Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesis

Author:

DeBruhl Heather1,Albertson Roger2,Swider Zachary1,Sullivan William1

Affiliation:

1. Molecular, Cell and Developmental Biology, University of California at Santa Cruz, Santa Cruz, CA 95064, USA

2. Biology Department, Albion College, Albion, MI 49224, USA

Abstract

Physically separating daughter cells during cytokinesis requires contraction of an actin-myosin ring and vesicle-mediated membrane addition at the cleavage furrow. To identify vesicle trafficking proteins that function in cytokinesis, we screened deficiencies and mutations of candidate genes by live-imaging the mitotic domains of the Drosophila embryo. In embryos homozygous for some of these deficiencies, we observed several cytokinesis phenotypes, including slow furrow ingression and increased membrane blebbing. We also found that cytokinesis required the Sec1/Munc18 homolog, Rop, which interacts with syntaxin and mediates exocytosis at the plasma membrane. In a temperature sensitive Rop mutant (RopTS), the contractile ring disassembled during furrow ingression, indicating that maintenance of the ring required vesicle addition. Furthermore, in some dividing RopTS cells, the shape of the daughter cells became unstable, causing cytokinesis failure. These results further highlight the importance of vesicle trafficking in animal cytokinesis and show that vesicle fusion influences cell shape during cytokinesis.

Funder

National Institutes of Health

Albion College

Publisher

The Company of Biologists

Subject

Cell Biology

Reference77 articles.

1. Membrane traffic: a driving force in cytokinesis;Albertson;Trends Cell Biol.,2005

2. Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle;Albertson;J. Cell Biol.,2008

3. The KEULE gene is involved in cytokinesis in Arabidopsis;Assaad;Mol. Gen. Genet.,1996

4. The cytokinesis gene KEULE encodes a Sec1 protein that binds the syntaxin KNOLLE;Assaad;J. Cell Biol.,2001

5. Region-specific effects on chromosome integrity of mutations at essential loci in Drosophila melanogaster;Baker;Proc. Natl. Acad. Sci. USA,1982

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