CsTFL1 inhibits determinate growth and terminal flower formation through interaction with CsNOT2a in cucumber (Cucumis sativus L.)

Author:

Wen Changlong12,Zhao Wensheng3,Liu Weilun1,Yang Luming2,Wang Yuhui2,Liu Xingwang3,Xu Yong1,Ren Huazhong3,Guo Yangdong3,Li Cong4,Li Jigang4,Weng Yiqun25,Zhang Xiaolan3ORCID

Affiliation:

1. Beijing Vegetable Research Center (BVRC), Beijing Academy of Agricultural and Forestry Sciences, Beijing Key Laboratory of Vegetable Germplasms Improvement, National Engineering Research Center for Vegetables, Beijing 100097, China

2. Department of Horticulture, University of Wisconsin-Madison, WI 53706, USA

3. Department of Vegetable Sciences, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, China

4. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China

5. USDA-ARS, Vegetable Crops Research Unit, 1575 Linden Drive, Madison, WI 53706, USA

Abstract

Cucumber is an important vegetable crop that carries on vegetative growth and reproductive growth simultaneously. Indeterminate growth is favorable for fresh market under protected environments, while determinate growth is preferred for pickling cucumber in the once-over mechanical harvest system. The genetic basis of determinacy is largely unknown in cucumber. In this study, map-based cloning of the de locus showed that the determinate growth habit is caused by a non-synonymous SNP in CsTFL1. CsTFL1 is expressed in the subapical regions of the shoot apical meristem, lateral meristem and young stems. Ectopic expression of CsTFL1 rescued the terminal flower phenotype in the Arabidopsis tfl1-11 mutant and delayed flowering in wild type Arabidopsis. Knockdown of CsTFL1 resulted in determinate growth and formation of terminal flowers in cucumber. Biochemical analyses indicated that CsTFL1 interacts with a homolog of miRNA biogenesis gene CsNOT2a, CsNOT2a interacts with FDP. Cucumber CsFT directly interacts with CsNOT2a and CsFD, and CsFD interacts with two 14-3-3 proteins. These data suggested that CsTFL1 competes with CsFT for interaction with CsNOT2a-CsFDP to inhibit determinate growth and terminal flower formation in cucumber.

Funder

National Natural Science Foundation of China

Beijing Municipal National Science Foundation

Beijing nova program

Beijing Municipal Science and Technology Commission

Beijing Academy of Agricultural & Forestry Sciences

Chinese Universities Scientific Fund

111 Project

National Institute of Food and Agriculture

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

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