Determinants of T box protein specificity
Author:
Conlon Frank L.12, Fairclough Lynne1, Price Brenda M. J.1, Casey Elena S.13, Smith J. C.14
Affiliation:
1. Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK 2. Present address: Department of Genetics, Fordham Hall, UNC-Chapel Hill, Chapel Hill, NC 27599-3280, USA 3. Present address; Department of Molecular Biology, University of California, Berkeley, CA 94720, USA 4. Present address: Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
Abstract
Members of the T box family of transcription factors play important roles in early development. Different members of the family exert different effects and here we show that much of the specificity of the Xenopus T box proteins Xbra, VegT and Eomesodermin resides in the DNA-binding domain, or T box. Binding site selection experiments show that the three proteins bind the same core sequence, but they select paired sites that differ in their orientation and spacing. Lysine 149 of Xbra is conserved in all Brachyury homologues, while the corresponding amino acid in VegT and Eomesodermin is asparagine. Mutation of this amino acid to lysine changes the inductive abilities of VegT and Eomesodermin to resemble that of Xbra.
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
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