Reduced thermal tolerance during salinity acclimation in brook trout (Salvelinus fontinalis) can be rescued by prior treatment with cortisol

Abstract

The aims of this study were to assess whether thermal tolerance of brook trout (Salvelinus fontinalis) is affected during seawater (SW) acclimation and to investigate the role of cortisol in osmoregulation and thermal tolerance during SW acclimation. Freshwater (FW)-acclimated brook trout at 18 °C (Tacc) were exposed to SW for 16 d, whilst maintaining a FW control. Fish were examined for critical thermal maximum (CTmax) 0 (before), 2, 5, and 16 d after SW exposure, and sampled at Tacc and CTmax for analysis of plasma cortisol, glucose, and Cl−, gill Na+/K+-ATPase (NKA) activity and heat shock protein 70 (HSP70) abundance, and white muscle water content. At 2 d in SW, CTmax was significantly reduced (from 31 to 26 °C), then recovered by 16 d. This transient decrease in thermal tolerance coincided with a transient increase in plasma Cl− and decrease in muscle moisture content. Salinity itself had no effect on gill HSP70 abundance compared to the large and immediate effects of high temperature exposure during CTmax testing. To examine the role of cortisol in osmoregulation, brook trout were administered a cortisol implant (5 and 25 µg/g CORT) prior to SW exposure. Both CORT doses significantly increased their capacity to maintain plasma Cl− during SW acclimation. Treatment with the 25 µg/g CORT dose was shown to significantly improve CTmax after 2 d in SW, and CTmax was associated with plasma Cl−. These findings indicate that brook trout are sensitive to temperature during SW acclimation and that thermal tolerance is associated with ion and water balance during SW acclimation.