Dma1-dependent degradation of Septation Initiation Network proteins during meiosis in Schizosaccharomyces pombe

Abstract

The Schizosaccharomyces pombe septation initiation network (SIN) is required for cytokinesis during vegetative growth and spore formation during meiosis. Regulation of the SIN during mitosis has been studied extensively, but less is known about its meiotic regulation. Here, we show that several aspects of the SIN regulation differ between mitosis and meiosis. First, the presence of GTP-bound spg1p is not the main determinant of the timing of cdc7p and sid1p association with the SPB during meiosis. Second, the localisation dependencies of SIN proteins differ from those in mitotic cells, suggesting a modified functional organisation of the SIN during meiosis. Third, there is stage-specific degradation of SIN components in meiosis; byr4p is degraded after meiosis I, while the degradation of cdc7p, cdc11p and sid4p occurs after the second meiotic division and depends upon the ubiquitin ligase dma1p. Finally, dma1p-dependent degradation is not restricted to the SIN, for we show that dma1p is needed for the degradation of mcp6p/hrs1p in meiosis I. Together, these data suggest that stage-specific targetted proteolysis will play an important role in regulating meiotic progression.