Affiliation:
1. Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, United Kingdom
Abstract
Non-coding Y RNAs are essential for the initiation of chromosomal DNA replication in vertebrates, yet their association with chromatin during the cell cycle is not characterised. Here, we quantify human Y RNA levels in soluble and chromatin-associated intracellular fractions and investigate topographically their dynamic association with chromatin during the cell cycle. We find that, on average, about a million Y RNA molecules are present in the soluble fraction of a proliferating cell, and 5-10-fold less in association with chromatin. These levels decrease substantially in quiescence. No significant differences are apparent between cancer and non-cancer cell lines. Y RNAs associate with euchromatin throughout the cell cycle. Their levels are 2-4-fold higher in S than in G1 phase or mitosis. Y RNAs are not detectable at active DNA replication foci, and re-associate with replicated euchromatin during mid/late S phase. The dynamics and sites of Y1 RNA association with chromatin are concordant with those of the origin recognition complex, ORC. Our data therefore suggest a functional role of Y RNAs in a common pathway with ORC.
Funder
Association for International Cancer Research
Wellcome Trust
Isaac Newton Trust
Publisher
The Company of Biologists
Cited by
11 articles.
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