Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation

Author:

Shi Lin1,Kong Ruiyan1,Li Zhengran1,Zhao Hang1,Ma Rui2,Bai Guang1,Li Jing1,Li Zhouhua1ORCID

Affiliation:

1. College of Life Sciences, Capital Normal University, Beijing 100048, China

2. Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Abstract

ABSTRACT Adult stem cells are critical for the maintenance of tissue homeostasis. However, how the proliferation and differentiation of intestinal stem cells (ISCs) are regulated remains not fully understood. Here, we find a mutant, stum 9-3, affecting the proliferation and differentiation of Drosophila adult ISCs in a forward genetic screen for factors regulating the proliferation and differentiation ISCs. stum 9-3 acts through the conserved Notch signaling pathway, upstream of the S2 cleavage of the Notch receptor. Interestingly, the phenotype of stum 9-3 mutant is not caused by disruption of stumble (stum), where the p-element is inserted. Detailed mapping, rescue experiments and mutant characterization show that stum 9-3 is a new allele of O-fucosyltransferase 1 (O-fut1). Our results indicate that unexpected mutants with interesting phenotype could be recovered in forward genetic screens using known p-element insertion stocks.

Funder

National Natural Science Foundation of China

Beijing Natural Science Foundation

Publisher

The Company of Biologists

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

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