Role of GnIH in photoperiodic regulation of seasonal reproduction in the Eurasian tree sparrow

Abstract

Seasonal reproductive cycles of most birds are regulated by photoperiod via neuroendocrine control. GnIH in hypothalamus has been reported to act as neuroendocrine integrator of photoperiodic cues. In this study, both captive and field investigations were carried out to understand the effects of photoperiod and seasonality on GnIH expression in subtropical tree sparrows. Monthly observations on GnIH mRNA and peptide expressions in wild birds over a year revealed significant increase in GnIH mRNA level and number of GnIH-ir neurons during the nonbreeding season when compared to their expressions in the breeding season. GnIH-ir neurons were found primarily in paraventricular nucleus (PVN) with their fibers projecting into median eminence and some other areas of brain. In an eight month long experiment, birds exposed to short days had higher GnIH expression compared to birds on long days regardless of sampling month. Long day birds with regressed testes had similar GnIH levels compared to short day birds. Though the number of GnIH peptide expressing neurons ran almost parallel to the levels of GnIH mRNA, they were found inversely related to gonadal size in both sexes under natural and artificial photoperiodic conditions. These results clearly indicate inhibitory role of GnIH in photoperiodic regulation of seasonal reproduction in the tree sparrow.