Evidence that water exudes when holothurian connective tissue stiffens

Abstract

SUMMARYThe dermis of the body wall of sea cucumbers is composed mainly of extracellular materials such as collagens, proteoglycans and water; the water content is as high as 80%. Yet it shows rapid changes in stiffness under neural control. The dermis has been proposed to assume three mechanical states, soft, standard, and stiff. We investigated the relationship between the stiffness and the dermal mass and volume. Both the mass and volume decreased by 15% when the dermis stiffened from the standard state to the stiff state by mechanical stimulation and by chemical stimulation with potassium-rich seawater. The effect of the latter was abolished by anesthesia. The mass decrease was caused largely by water exudation. Tensilin, a holothurian protein that stiffens the soft dermis to form the standard state, did not cause any changes in mass. These results suggested that the stiffening mechanisms responsible for the transition from the soft state to the standard state, and that from the standard to the stiff state, are different. The removal of water from the dermis in the standard state, by soaking in hypertonic solution, caused only slight stiffening, which suggested that water exudation was not the direct cause of the stiffening. A change of pH of the surrounding medium, either more acidic or basic, was not associated with mass changes, although it caused a large increase in stiffness. The implications of the present results for the molecular mechanisms of the stiffness changes are discussed.