Branching gene regulatory network dictating different aspects of a neuronal cell identity

Abstract

The nervous system displays a daunting cellular diversity. Neuronal sub-types differ from each other in several aspects, including their neurotransmitter expression and axon projection. These aspects can converge, but can also diverge, such that neurons expressing the same neurotransmitter may project axons to different targets. It is not well understood how regulatory programs converge/diverge to associate/dissociate different cell fate features. Studies of the Drosophila Tv1 neurons have identified a regulatory cascade; ladybird early -> collier -> apterous/eyes absent -> dimmed, which specifies Tv1 neurotransmitter expression. Here, we conduct genetic and transcriptome analysis to address how other aspects of Tv1 cell fate is governed. We find that an initiator terminal selector gene triggers a feedforward loop which branches into different subroutines, each of which establishes different features of this one unique neuronal cell fate.