Reconstitution of cell migration at a glance

Author:

Garcia-Arcos Juan Manuel12,Chabrier Renaud3,Deygas Mathieu12,Nader Guilherme12,Barbier Lucie12,Sáez Pablo José12,Mathur Aastha12,Vargas Pablo12,Piel Matthieu12ORCID

Affiliation:

1. Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France

2. Institut Pierre-Gilles de Gennes, PSL Research University, F-75005 Paris, France

3. Institut Curie, PSL Research University, CNRS UMR3348, F-91405 Orsay, France

Abstract

ABSTRACT Single cells migrate in a myriad of physiological contexts, such as tissue patrolling by immune cells, and during neurogenesis and tissue remodeling, as well as in metastasis, the spread of cancer cells. To understand the basic principles of single-cell migration, a reductionist approach can be taken. This aims to control and deconstruct the complexity of different cellular microenvironments into simpler elementary constrains that can be recombined together. This approach is the cell microenvironment equivalent of in vitro reconstituted systems that combine elementary molecular players to understand cellular functions. In this Cell Science at a Glance article and accompanying poster, we present selected experimental setups that mimic different events that cells undergo during migration in vivo. These include polydimethylsiloxane (PDMS) devices to deform whole cells or organelles, micro patterning, nano-fabricated structures like grooves, and compartmentalized collagen chambers with chemical gradients. We also outline the main contribution of each technique to the understanding of different aspects of single-cell migration.

Publisher

The Company of Biologists

Subject

Cell Biology

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