Dietary phosphate affects food selection, post-ingestive P fate, and performance of a polyphagous herbivore

Abstract

Comparisons of the carbon, nitrogen, and phosphorus contents of plants and insect herbivores suggest that phosphorus (P) limitation and herbivore foraging to balance P intake could be common. However, lack of synthetic diets for testing the effects of lower ranges of dietary P has been a major impediment to experimental assessment of the ecological importance of, and physiological responses to, P limitation for terrestrial herbivores. We manipulated dietary P content (% P) over its observed range in terrestrial foliage using artificial diets containing near-optimal contents of other nutrients for the grasshopper Schistocerca americana. Over much of the ecologically relevant range, when consuming single diets over a lifetime, higher P stimulated growth rates and increased survival, with an optimal dietary % P of 0.25-0.50% when measured throughout development. Excessive dietary P (1%) reduced growth and survival. However, with only short-term (3 day) confinement to single diets, dietary P had no effect on food consumption or growth rates. During these short exposures, fifth (but not third) instar hoppers increased the proportion of P excreted relative to P assimilated as dietary P increased. Target experiments demonstrated that, when given a choice, grasshoppers select among foods to attain a P intake target of 0.6%. These data suggest that P-limitation could be common for terrestrial insect herbivores and that they can exhibit ingestive and post-ingestive mechanisms to attain sufficient but not excessive P.