A scanning electron-microscopic study of the local degeneration of cilia during sexual reproduction in Paramecium

Abstract

The location and extent of local degeneration of cilia during sexual reproduction of Paramecium was studied using scanning electron microscopy to examine cells undergoing conjugation and autogamy. At some time during the mating reaction, but prior to conjugant pair formation, ciliary degeneration begins at the antero-ventral tip of cells and proceeds posteriorly along the suture. In the anterior part of the cell, degeneration occurs on both sides of the suture, but in the posterior part it is restricted to the right side of the suture. In 5 species of Paramecium examined, degeneration occurred in nearly the same region. No degeneration of cilia is observed in natural autogamy of P. tetraurelia, whereas in chemically induced autogamy of P. caudatum degeneration occurs as in ordinary conjugation. Conjugant pairs never expose any deciliated cell surface except at the postero-ventral tip. The maximum extent of ciliary degeneration is best seen in the chemically induced autogamous cells: 7 kinetics (rows of unit teritories) at the anterior-left, 4 kinetics at the anterior-right, 10 or more kinetics at the posterior-right and the right wall of the vestibule of the mouth. Before complete disappearance of the cilia, many short cilia are observed. This suggests that ciliary degeneration is due to resorption. Degeneration extends more rapidly in cells with stronger mating reactivity. The relations between mating reactivity, ciliary degeneration and nuclear activation are discussed.