Satellite DNA binding and cellular localisation of RNA helicase P68-Reference-Cited by-同舟云学术

Satellite DNA binding and cellular localisation of RNA helicase P68

Published:2005-02-01 Issue:3 Volume:118 Page:611-622
ISSN:1477-9137
Container-title:Journal of Cell Science
language:en
Short-container-title:

Author:

Enukashvily Natella¹,Donev Rossen²,Sheer Denise²,Podgornaya Olga¹

Affiliation:

1. Cell Cultures Department, Institute of Cytology, Tikhoretsky, 4, St Petersburg, 194064, Russia

2. Human Cytogenetics Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields, London WC2A 3PX, UK

Abstract

We purified a 68-kDa protein from the mouse nuclear matrix using ion exchange and affinity chromatography. Column fractions were tested for specific binding to mouse minor satellite DNA using a gel mobility shift assay. The protein was identified by mass spectrometry as RNA helicase P68. In fixed cells, P68 was found to shuttle in and out of SC35 domains, forming fibres and granules in a cell-cycle dependent manner. Analysis of the P68 sequence revealed a short potential coiled-coil domain that might be involved in the formation of P68 fibres. Contacts between centromeres and P68 granules were observed during all phases of the cycle but they were most prominent in mitosis. At this stage, P68 was found in both the centromeric regions and the connections between chromosomes. Direct interaction of P68/DEAD box RNA helicase with satellite DNAs in vitro has not been demonstrated for any other members of the RNA helicase family.

Publisher

The Company of Biologists

Subject

Cell Biology

Link

http://journals.biologists.com/jcs/article-pdf/118/3/611/1522668/611.pdf

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