Optimized CUBIC protocol for 3D imaging of chicken embryos at single-cell resolution-Reference-Cited by-同舟云学术

Optimized CUBIC protocol for 3D imaging of chicken embryos at single-cell resolution

Published:2017-01-01 Issue: Volume: Page:
ISSN:1477-9129
Container-title:Development
language:en
Short-container-title:

Author:

Gómez-Gaviro María Victoria¹²³^ORCID,Balaban Evan⁴⁵,Bocancea Diana¹,Lorrio María Teresa¹,Pompeiano María⁴⁵,Desco Manuel¹²³^ORCID,Ripoll Jorge¹²^ORCID,Vaquero Juan José¹²^ORCID

Affiliation:

1. Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain

2. Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

3. Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Madrid, Spain

4. Department of Psychology, McGill University, Montreal, Canada

5. Current address: Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain

Abstract

The CUBIC tissue clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell-level resolution in whole early-stage chicken embryos, and in the whole brains of late-stage embryos.

Funder

Human Frontier Science Program

Ministerio de Economía y Competitividad

Consejería de Educación, Juventud y Deporte, Comunidad de Madrid

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

Link

http://journals.biologists.com/dev/article-pdf/144/11/2092/1847367/dev145805.pdf

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