Cell death and renewal during prey capture and digestion in the carnivorous spongeAsbestopluma hypogea(Porifera: Poecilosclerida)

Abstract

SummaryThe sponge Asbestopluma hypogea is unusual among sponges due to its peculiar carnivorous feeding habit. During various stages of its nutrition cycle, the sponge is subjected to spectacular morphological modifications. Starved animals are characterized by many elongated filaments which are crucial for the sponge to capture prey. After capture, and during the digestion process, these filaments actively regress before being regenerated during a subsequent period of starvation. Here, we demonstrate that these morphological events repose on a highly dynamic cellular turnover implying a coordinated sequence of programmed cell death (apoptosis and autophagy), cell proliferation and cell migration. A candidate niche for cell renewal by stem cell proliferation and differentiation was identified at the base of the sponge peduncle, characterized by surpassing levels of BrdU/EdU incorporation. Therefore, BrdU/EdU positive-cells of the peduncle base are candidate motile cells responsible for the regeneration of the prey-capturing main sponge body, i.e. the dynamic filaments. Altogether, our results demonstrate that dynamic of cell renewal in sponge appears to be regulated by cellular mechanisms as multiple and complex as those already identified in bilaterian metazoans.