Cement gland-specific activation of the Xag1 promoter is regulated by co-operation of putative Ets and ATF/CREB transcription factors

Author:

Wardle Fiona C.1,Wainstock Daniel H.2,Sive Hazel L.1

Affiliation:

1. Present address: Wellcome Trust/Cancer Research UK Institute, Tennis Court Road, Cambridge CB2 1QR, UK

2. Whitehead Institute for Biomedical Research, and Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA

Abstract

The cement gland marks the extreme anterior ectoderm of the Xenopus embryo, and is determined through the overlap of several positional domains. In order to understand how these positional cues activate cement gland differentiation, the promoter of Xag1, a marker of cement gland differentiation, was analyzed. Previous studies have shown that Xag1 expression can be activated by the anterior-specific transcription factor Otx2, but that this activation is indirect. 102 bp of upstream genomic Xag1 sequence restricts reporter gene expression specifically to the cement gland. Within this region, putative binding sites for Ets and ATF/CREB transcription factors are both necessary and sufficient to drive cement gland-specific expression, and cooperate to do so. Furthermore, while the putative ATF/CREB factor is activated by Otx2, a factor acting through the putative Ets-binding site is not. These results suggest that Ets-like and ATF/CREB-like family members play a role in regulating Xag1 expression in the cement gland, through integration of Otx2 dependent and independent pathways.

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

Reference48 articles.

1. Acampora, D., Mazan, S., Lallemand, Y., Avantaggiato, V., Maury, M., Simeone, A. and Brulet, P. (1995). Forebrain and midbrain regions are deleted in Otx2–/– mutants due to a defective anterior neuroectoderm specification during gastrulation. Development121, 3279-3290.

2. Ang, S. L., Jin, O., Rhinn, M., Daigle, N., Stevenson, L. and Rossant, J. (1996). A targeted mouse Otx2 mutation leads to severe defects in gastrulation and formation of axial mesoderm and to deletion of rostral brain. Development122, 243-252.

3. Baltzinger, M., Mager-Heckel, A. M. and Remy, P. (1999). Xlerg: expression pattern and overexpression during development plead for a role in endothelial cell differentiation. Dev. Dyn.216, 420-433.

4. Blitz, I. L. and Cho, K. W. Y. (1995). Anterior neurectoderm is progressively induced during gastrulation: the role of the Xenopus homeobox gene orthodenticle. Development121, 993-1004.

5. Bradley, L., Wainstock, D. and Sive, H. (1996). Positive and negative signals modulate formation of the Xenopus cement gland. Development122, 2739-2750.

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