Phosphorylation-dependent routing of RLP44 towards brassinosteroid or phytosulfokine signalling

Author:

Garnelo Gómez Borja12,Holzwart Eleonore1,Shi Chaonan2,Lozano-Durán Rosa23,Wolf Sebastian13ORCID

Affiliation:

1. Centre for Organismal Studies Heidelberg, University of Heidelberg, INF230, 69120 Heidelberg, Germany

2. Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences (CAS), Shanghai 201602China

3. Department of Plant Biochemistry, Centre for Plant Molecular Biology (ZMBP), Eberhard Karls University, D-72076 Tübingen, Germany

Abstract

ABSTRACT Plants rely on cell surface receptors to integrate developmental and environmental cues into behaviour adapted to the conditions. The largest group of these receptors, leucine-rich repeat receptor-like kinases, form a complex interaction network that is modulated and extended by receptor-like proteins. This raises the question of how specific outputs can be generated when receptor proteins are engaged in a plethora of promiscuous interactions. RECEPTOR-LIKE PROTEIN 44 (RLP44) acts to promote both brassinosteroid and phytosulfokine signalling, which orchestrate diverse cellular responses. However, it is unclear how these activities are coordinated. Here, we show that RLP44 is phosphorylated in its highly conserved cytosolic tail and that this post-translational modification governs its subcellular localization. Whereas phosphorylation is essential for brassinosteroid-associated functions of RLP44, its role in phytosulfokine signalling is not affected by phospho-status. Detailed mutational analysis suggests that phospho-charge, rather than modification of individual amino acids determines routing of RLP44 to its target receptor complexes, providing a framework to understand how a common component of different receptor complexes can get specifically engaged in a particular signalling pathway.

Funder

Deutsche Forschungsgemeinschaft

Eberhard Karls Universität Tübingen

Publisher

The Company of Biologists

Subject

Cell Biology

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