Affiliation:
1. A.R.C. Unit of Invertebrate Chemistry and Physiology, Department of Zoology, Downing Street, Cambridge and Department of Pharmacology, Hills Road, Cambridge
Abstract
1. The sites of the transepithelial potential changes produced by 5-HT and cyclic AMP in the salivary gland of the blowfly (Calliphora) have been investigated.
2. Microelectrodes recorded a two-step potential profile across this epithelium. The cell was negative to both the bathing medium and the saliva.
3. The basal membrane potential was 44·0±0·2 mV and was affected very little by application of either 5-HT or cyclic AMP.
4. The apical membrane was depolarized by 5-HT and in contrast was hyperpolarized by cyclic AMP.
5. On the basis of these results it is concluded that 5-HT has two actions: (a) to stimulate a potassium pump on the apical membrane using cyclic AMP as an intermediary, (b) to increase chloride movement by a mechanism not directly involving cyclic AMP.
Publisher
The Company of Biologists
Subject
Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics
Cited by
42 articles.
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