Iodine accumulation in sea urchin larvae is dependent on peroxide

Abstract

Summary Iodine has many important biological functions and its concentrations vary with environment. Recent research has provided novel insights into iodine uptake mechanisms in marine bacteria and kelp through hydrogen peroxide dependent diffusion (PDD). This mechanism is distinct from sodium dependent mechanisms known from vertebrates. In vertebrates, iodine accumulates in the thyroid gland by the action of the apical iodide transporter (AIT) and the sodium iodide symporter (NIS). Neither of these proteins has, thus far, been identified outside of the chordates and PDD (as an iodine uptake mechanism) has never been studied in animals. Using 125I as a marker for total iodine influx, we tested iodine uptake via sodium dependent transport versus PDD in embryos and larvae of the sea urchin Strongylocentrotus purpuratus. We found that iodine uptake in S. purpuratus is largely independent of NIS/AIT. Instead we found that uptake is dependent on the presence and production of hydrogen peroxide indicating that sea urchin larvae use PDD as a mechanism for iodine acquisition. Our data, for the first time, provide conclusive evidence for this mechanism in an animal. Furthermore, our data strongly suggest that sodium dependent iodine uptake via active transporter proteins is a synapomorphy of chordates.