Sound production by abdominal tymbal organs in two moth species: the green silver-line and the scarce silver-line (Noctuoidea: Nolidae: Chloephorinae)

Abstract

Male moths of the chloephorine species Pseudoips prasinana and Bena bicolorana produce clicks (approximately 100 dB peSPL at 10 cm) using ventral tymbal organs located in a cleft in the second abdominal sternite. Large muscles insert on the dorsal part of the tymbal frame and rhythmically flex a thin sheet of cuticle. Normally, each sound-production cycle contains four clicks, the left and right tymbals producing clicks both on active buckling caused by muscle contraction and on the passive elastic return from buckling. Histochemical staining indicated the presence of elastic resilin-like proteins in the tymbals. Obvious differences between the click patterns of the two species reflect differences in their tymbal morphology. P. prasinana has smooth tymbals and produces a single click (300?s, 40 kHz) for each tymbal buckling. In contrast, B. bicolorana has striae on the medial part of the tymbals. Accordingly, it produces many clicks per buckling. The click pattern is a heterogeneous mixture of large clicks at 52 kHz, resembling those of P. prasinana, interspersed with series of broad-band clicks (20–100 kHz) of lower intensity (15–20 dB). Thus, in chloephorine moths, there is a correlation between the structure and function of the smooth and striated tymbals that is strikingly similar to that in arctiid moths, although the two types of tymbals have evolved independently. The hearing of P. prasinana is tuned to its own sounds with lowest threshold (38 dB SPL) at 40–60 kHz. We suggest that sound production in male chloephorines plays a part in sexual acoustic communication.