The centralspindlin complex regulates cytokinesis and morphogenesis in the C. elegans spermatheca

Author:

Zhang Pei12,Chen Jiwei12,Wang Xiangchuan12,Geng Yingchao12,Sun Liangyu3,Zhang Hongjie124ORCID

Affiliation:

1. Centre of Reproduction, Development and Aging 1 , Faculty of Health Sciences , , Taipa, Macau SAR 999078 , China

2. University of Macau 1 , Faculty of Health Sciences , , Taipa, Macau SAR 999078 , China

3. University of Macau 2 Biological Imaging and Stem Cell Core, Faculty of Health Sciences , , Taipa, Macau SAR 999078 , China

4. MoE Frontiers Science Center for Precision Oncology, University of Macau 3 , Taipa, Macau SAR 999078 , China

Abstract

ABSTRACT Organ morphogenesis needs orchestration of a series of cellular events, including cell division, cell shape change, cell rearrangement and cell death. Cytokinesis, the final step of cell division, is involved in the control of organ size, shape and function. Mechanistically, it is unclear how the molecules involved in cytokinesis regulate organ size and shape. Here, we demonstrate that the centralspindlin complex coordinates cell division and epithelial morphogenesis by regulating cytokinesis. Loss of the centralspindlin components CYK-4 and ZEN-4 disrupts cell division, resulting in altered cell arrangement and malformation of the Caenorhabditis elegans spermatheca. Further investigation revealed that most spermathecal cells undergo nuclear division without completion of cytokinesis. Germline mutant-based analyses suggest that CYK-4 regulates cytokinesis of spermathecal cells in a GTPase activator activity-independent manner. Spermathecal morphology defects can be enhanced by double knockdown of rho-1 and cyk-4, and partially suppressed by double knockdown of cdc-42 and cyk-4. Thus, the centralspindlin components CYK-4 and ZEN-4, together with RHO-1 and CDC-42, are central players of a signaling network that guides spermathecal morphogenesis by enabling completion of cytokinesis.

Funder

Science and Technology Development Fund

Fundo para o Desenvolvimento das Ciências e da Tecnologia

Universidade de Macau

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

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