The ecl family gene ecl3+ is induced by phosphate starvation and contributes to sexual differentiation in fission yeast

Author:

Ohtsuka Hokuto1ORCID,Sakata Hiroki1,Kitazaki Yuto1,Tada Masanobu1,Shimasaki Takafumi1ORCID,Otsubo Yoko234ORCID,Maekawa Yasukichi1,Kobayashi Mikuto1,Imada Kazuki56ORCID,Yamashita Akira27ORCID,Aiba Hirofumi1ORCID

Affiliation:

1. Graduate School of Pharmaceutical Sciences, Nagoya University 1 Laboratory of Molecular Microbiology , , Nagoya, Aichi 464-8601 , Japan

2. Interdisciplinary Research Unit, National Institute for Basic Biology 2 , Okazaki, Aichi 444-858 , Japan

3. National Institute for Fusion Science 3 , Toki, Gifu 509-5292 , Japan

4. Center for Novel Science Initiatives, National Institutes of Natural Sciences 4 , Okazaki, Aichi 444-8585 , Japan

5. National Institute of Technology (KOSEN), Suzuka College 5 Department of Chemistry and Biochemistry , , Suzuka 510-0294 , Japan

6. Graduate School of Science, Osaka City University 6 Department of Biology , , Sumiyoshi-ku, Osaka 558-8585 , Japan

7. Center for Low-temperature Plasma Sciences, Nagoya University 7 , Nagoya, Aichi 464-8603 , Japan

Abstract

ABSTRACT In Schizosaccharomyces pombe, ecl family genes are induced by several signals, such as starvation of various nutrients, including sulfur, amino acids and Mg2+, and environmental stress, including heat or oxidative stress. These genes mediate appropriate cellular responses and contribute to the maintenance of cell viability and induction of sexual differentiation. Although this yeast has three ecl family genes with overlapping functions, any environmental conditions that induce ecl3+ remain unidentified. We demonstrate that ecl3+ is induced by phosphate starvation, similar to its chromosomally neighboring genes, pho1+ and pho84+, which respectively encode an extracellular acid phosphatase and an inorganic phosphate transporter. ecl3+ expression was induced by the transcription factor Pho7 and affected by the cyclin-dependent kinase (CDK)-activating kinase Csk1. Phosphate starvation induced G1 arrest and sexual differentiation via ecl family genes. Biochemical analyses suggested that this G1 arrest was mediated by the stabilization of the CDK inhibitor Rum1, which was dependent on ecl family genes. This study shows that ecl family genes are required for appropriate responses to phosphate starvation and provides novel insights into the diversity and similarity of starvation responses.

Funder

Japan Society for the Promotion of Science

Naito Foundation

Nagoya University

Publisher

The Company of Biologists

Subject

Cell Biology

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