Diverse dose-response effects of yolk androgens on embryo development and nestling growth in a wild passerine

Abstract

Avian egg yolks contain various amounts of maternally derived androgens that can modify offspring phenotype and adjust their development to posthatching environment. Seemingly adaptive variation in yolk androgen levels with respect to breeding density conditions or male attractiveness has been found in numerous studies. One important consideration that has been overlooked in previous research is the likely non-linear nature of hormone effects. To examine possible complex dose-response effects of maternal androgens on chick development, we experimentally administered three different androgen doses of the naturally-occurring mixture of yolk testosterone and androstenedione to spotless starling eggs (Sturnus unicolor). We found that yolk androgens show a non-linear dose-response pattern for several traits. Thus, androgens had a stimulatory effect on hatching body mass and nestling skeletal growth, but maximum values were found at intermediate doses, whereas our highest dose resulted in a decrease. However, the opposite U-shaped effect was found on nestling body mass. We also detected linear negative and positive effects on embryonic development period and nestling gape width, respectively. Our results suggest differential tissue responsiveness to yolk androgens, which may result in compromises in maternal allocation to produce adapted phenotypes. Due to the non-linear dose-response pattern, future investigations should carefully consider a wide range of concentrations, since the balance of costs and benefits may strongly differ depending on concentration.