Author:
Aihara Masamune,Jin Xiulian,Kurihara Yusuke,Yoshida Yutaka,Matsushima Yuichi,Oku Masahide,Hirota Yuko,Saigusa Tetsu,Aoki Yoshimasa,Uchiumi Takeshi,Yamamoto Tadashi,Sakai Yasuyoshi,Kang Dongchon,Kanki Tomotake
Abstract
When mitophagy is induced in Saccharomyces cerevisiae, the mitochondrial outer membrane protein ScAtg32 interacts with the cytosolic adaptor protein ScAtg11. ScAtg11 then delivers the mitochondria to the pre-autophagosomal structure for autophagic degradation. Despite the importance of ScAtg32 for mitophagy, the expression and functional regulation of ScAtg32 are poorly understood. In this study, we identified and characterized the ScAtg32 homolog in Pichia pastoris (PpAtg32). Interestingly, we found that PpAtg32 was barely expressed before induction of mitophagy and was rapidly expressed after induction of mitophagy by starvation. Additionally, PpAtg32 was phosphorylated when mitophagy was induced. We found that PpAtg32 expression was suppressed by Tor and the downstream PpSin3-PpRpd3 complex. Inhibition of Tor by rapamycin induced PpAtg32 expression, but could neither phosphorylate PpAtg32 nor induce mitophagy. Based on these findings, we conclude that the Tor and PpSin3-PpRpd3-related pathway regulates PpAtg32 expression, but not PpAtg32 phosphorylation.
Publisher
The Company of Biologists
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