Cytokinin signalling regulates organ identity via AHK4 receptor in Arabidopsis

Abstract

Mutual interactions of the phytohormones cytokinins and auxin determine root or shoot identity during postembryonic de novo organogenesis in plants. However, our understanding to the role of hormonal metabolism and perception during early stages of cell fate reprograming is still elusive. In the hypocotyl explant assay, auxin activated root formation while cytokinins mediated early loss of the root identity, primordia disorganization and initiation of shoot development. Exogenous but also endogenous cytokinins influenced the initiation of newly formed organs as well as the pace of organ developmental sequence. The process of de novo shoot apical meristem establishment was accompanied by accumulation of endogenous cytokinins, differential regulation of genes for individual cytokinin receptors, strong activation of AHK4-mediated signalling and induction of shoot-specific homeodomain regulator WUSCHEL. The latter associated with upregulation of isopentenyladenine-type cytokinins, revealing higher shoot-forming potential when compared with trans-zeatin. Moreover, AHK4-controlled cytokinin signalling negatively regulated root stem cell organizer WUSCHEL RELATED HOMEOBOX 5 in the root quiescent centre. We propose an important role of endogenous cytokinin biosynthesis and AHK4-mediated cytokinin signalling in the control of de novo induced organ identity.