Ablation of connexin43 in uterine smooth muscle cells of the mouse causes delayed parturition-Reference-Cited by-同舟云学术

Ablation of connexin43 in uterine smooth muscle cells of the mouse causes delayed parturition

Published:2006-05-01 Issue:9 Volume:119 Page:1715-1722
ISSN:1477-9137
Container-title:Journal of Cell Science
language:en
Short-container-title:

Author:

Döring Britta¹,Shynlova Oksana²,Tsui Prudence²,Eckardt Dominik¹,Janssen-Bienhold Ulrike³,Hofmann Franz⁴,Feil Susanne⁴,Feil Robert⁴⁵,Lye Stephen J.²⁶⁷,Willecke Klaus¹

Affiliation:

1. Institut für Genetik, Abteilung Molekulargenetik, Universität Bonn, Römerstr. 164, 53117 Bonn, Germany

2. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

3. Neurobiologie, Carl von Ossietzky Universität Oldenburg, Oldenburg, Germany

4. Institut für Pharmakologie und Toxikologie, Technische Universität München, München, Germany

5. Interfakultäres Institut für Biochemie, Universität Tübingen, Tübingen, Germany

6. Institute of Medical Science, University of Toronto, Toronto, ON, Canada

7. Departments of Obstetrics and Gynecology and Physiology, University of Toronto, Toronto, ON, Canada

Abstract

Gap junctions are characteristically increased in the myometrium during term and preterm delivery and are thought to be essential for the development of uterine contractions during labour. Expression of connexin43 (Cx43), the major myometrial gap junction protein, is increased during delivery. We have generated a mouse mutant (Cx43fl/fl:SM-CreERT2), in which the coding region of Cx43 can be specifically deleted in smooth muscle cells at any given time point by application of tamoxifen. By this approach, we were able to study long-term effects on myometrial functions that are necessary for parturition as well as gap junction intercellular communication in primary myometrial cell cultures. We found a prolongation of the pregnancy in 82% of tamoxifen-treated Cx43fl/fl:SM-CreERT2 mice as well as decreased dye coupling in cultured primary myocytes of these animals. Other parturition-specific parameters such as the regulation of oxytocin receptor, prostaglandin F receptor or progesterone remained unchanged. Our results indicate the important function of Cx43 during parturition in the living animal and suggest further strategies to investigate the role of connexins in uterine contractility in transgenic mice.

Publisher

The Company of Biologists

Subject

Cell Biology

Link

http://journals.biologists.com/jcs/article-pdf/119/9/1715/1369241/1715.pdf

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