Survival in vitro of motoneurons identified or purified by novel antibody-based methods is selectively enhanced by muscle-derived factors

Author:

Bloch-Gallego E.1,Huchet M.1,el M'Hamdi H.1,Xie F.K.1,Tanaka H.1,Henderson C.E.1

Affiliation:

1. UPR 8402 du CNRS-U 249 de l'INSERM, Montpellier, France.

Abstract

Motoneurons were identified in vitro by a new method using the SC1 monoclonal antibody. They constituted up to 30% of total neurons in cultures of whole spinal cord from 4.5-day chicken embryos, and survived for at least 5 days in the presence of muscle extract, but not in its absence. By contrast, other neurons and floor-plate cells survived without muscle-derived factors. Motoneurons were purified to homogeneity by ‘panning’ on dishes coated with SC1 antibody; they developed rapidly even in the absence of other spinal cells. Concentrations of muscle extract required for half-maximal motoneuron survival were indistinguishable in pure and mixed cultures, suggesting that muscle-derived factors act directly on motoneurons. Other purified growth factors tested, including ciliary neurotrophic factor, did not have the survival-promoting activity of muscle.

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

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