Isolation of a residual protein structure from nuclei of the myxomycete Physarum polycephalum

Author:

Mitchelson K. R.1,Bekers A. G. M.1,Wanka F.1

Affiliation:

1. University of Nijmegen, Toernooiveld Department of Chemical Cytology , , Nijmegen, The Netherlands

Abstract

ABSTRACT A nuclear framework structure has been obtained from isolated interphase nuclei of Physarum polycephalum by extraction with 2·5 M NaCl and subsequent digestion with DNase. Wholemount electron micrographs showed a nuclear lamina containing residual pore structures associated with the fibrous internal matrix. The matrix was continuous with fibrillar remnants of the nucleolus. The structure was shown to consist of 2 major polypeptides of 23000 and 36 500 Daltons as well as 30 to 40 minor polypeptides of various molecular weight classes. The 2 major polypeptides were also prominent in preparations of the residual nucleolar material, suggesting that matrix proteins are common to both structures. The predominance of low-molecular-weight polypeptides in Physarum nuclear matrix suggests that there may be significant differences in composition of nuclear structural proteins between lower and higher eukaryotes.

Publisher

The Company of Biologists

Subject

Cell Biology

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