Adult-larvae vibrational communication in paper wasps: the role of abdominal wagging in Polistes dominula

Abstract

Communication through vibrational signals is widespread among social insects and regulates crucial social activities. Females of the social wasp Polistes dominula (Christ, 1791) produce substrate-borne vibrations on the combs by performing a conspicuous abdominal oscillatory behavior, known as abdominal wagging. Several studies have reported correlative evidence in support of its signaling role, but direct evidence is still lacking. Because abdominal wagging is strictly associated with the presence of larvae in the nest and with cell inspection, it has been suggested that it could be involved in adult–larvae communication. According to this hypothesis, abdominal wagging vibrations would have short-term effects related to food and trophallactic exchanges between adult and larvae by modulating salivary secretion (decreasing its amount, to prepare larvae to receive food, or stimulating the release of larval saliva to adults). Here, by using an electro-magnetic shaker, we assessed, for the first time, the short-term effect of abdominal wagging on larval behavior by recording larval response and by measuring the amount of saliva released immediately after abdominal wagging playback. Our results show that larvae are able to perceive the substrate-borne vibrations produced by abdominal wagging and react by increasing the movement of their body, possibly in order to attract the attention of adult females during feeding nest inspection. Yet, we found that vibrations neither increase nor decrease the release of larval saliva. Our results support the hypothesis of the alleged role of vibrations in adult–larvae communications; however, they do not support the long-lasting hypothesis of salivary release modulation.