Spectral and ultraviolet-polarisation sensitivity in juvenile salmonids: a comparative analysis using electrophysiology

Abstract

Spectral and polarisation sensitivity were compared among juvenile (parr) rainbow trout (Oncorhynchus mykiss), steelhead (O. mykiss), cutthroat trout (O. clarki clarki), kokanee (O. nerka) and brook char (Salvelinus fontinalis) using multi-unit recording from the optic nerve. Although reared under the same conditions, differences in photopic spectral sensitivity were evident. Specifically, ON-responses were co-dominated by L- and M-cone mechanisms in all fish except O. nerka, consistent with an M-cone mechanism sensitivity. The sensitivity of OFF-responses was dominated by the M-cone mechanism for all fish, but O. mykiss appeared to show an additional contribution from the L-cone mechanism. Using chromatic adaptation, an independent ultraviolet-sensitive mechanism is described for the first time for the salmonid genus Salvelinus. In addition, this ultraviolet-cone mechanism was present in the members of the genus Oncorhynchus that were examined. Thus, ultraviolet sensitivity appears to be common to the major extant clades of the subfamily Salmoninae. All species showed differential sensitivity to both vertical and horizontal linearly polarised light. This sensitivity differed between ON- and OFF-responses. The ON-responses were maximally sensitive to both vertically and horizontally polarised light, whereas the OFF-responses displayed maximal sensitivity to horizontally polarised light in all species, with reduced sensitivity to vertically polarised light compared with ON-responses. Because of the similarity in the physiological characteristics of polarisation sensitivity among the salmonid species examined, no relationship between the degree of migratory tendency and the ability to detect polarised light could be identified.