Integumentary L-histidine transport in a euryhaline polychaete worm: regulatory roles of calcium and cadmium in the transport event

Abstract

Integumentary uptake of L-[(3)H]histidine by polychaete worms (Nereis succinea) from estuarine waters of Oahu, Hawaii was measured in the presence and absence of calcium and cadmium using a physiological saline that approximated the ion composition of 60 % sea water. In this medium 1 micromol L(−1) cadmium significantly increased (P<0.01) the uptake of 10 micromol L(−1)L-[(3)H]histidine, while 1 micromol L(−1) cadmium plus 25 micromol L(−1)L-leucine significantly decreased (P<0.01) amino acid uptake. L-[(3)H]histidine influx was a sigmoidal function (n=2. 21+/−0.16, mean +/− s.e.m.) of [L-histidine] (1?50 micromol L(−1)) in the absence of cadmium, but became a hyperbolic function with the addition of 1 micromol L(−1) cadmium. A decrease of calcium concentration from 6 to 0 mmol L(−1) (lithium substitution) significantly increased (P<0.01) amino acid influx in the presence and absence of cadmium. Calcium significantly reduced (P<0.01), and cadmium significantly increased (P<0.01), L-[(3)H]histidine influx J(max), without either divalent cation affecting amino acid influx K(t). Variation in external sodium concentration (0?250 mmol L(−1)) had no effect on 10 micromol L(−1)L-[(3)H]histidine influx, but amino acid entry was a sigmoidal function of both [cadmium] (n=2.34+/−0.44) and [lithium] (n=1.91+/−0.39) in the absence of calcium. A model is proposed for transapical L-[(3)H]histidine influx by a transporter that resembles the classical sodium-independent L-system carrier protein that is regulated by the external divalent cations calcium and cadmium.