Regulation of proton transport in urinary epithelia

Author:

Al-Awqati Q.,Gluck S.,Reeves W.,Cannon C.

Abstract

In urinary epithelia, like the turtle bladder, protons are transported by a H+ translocating ATPase located in the luminal membrane. We have recently discovered that the H+ pump is stored in small vesicles that lie underneath the luminal membrane. CO2, a major regulator of H+ transport causes these vesicles to fuse with the membrane thereby inserting more H+ pumps. We have now isolated these vesicles from the turtle bladder and from beef kidney medulla. Based on inhibitor sensitivity and substrate specificity this proton translocating ATPase is different from the mitochondrial F0-F1 ATPase, yeast plasma membrane and the gastric H+,K+-ATPase. Solubilization and reconstitution of the enzyme into liposomes shows retention of transport activity and inhibitor sensitivity.

Publisher

The Company of Biologists

Subject

Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics

Cited by 12 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. The V-ATPase in insect epithelia;Journal of Experimental Biology;2017-09-15

2. New insights into the dynamic regulation of water and acid-base balance by renal epithelial cells;American Journal of Physiology-Cell Physiology;2012-05-15

3. H+-ATPase of crude homogenate of the outer mantle epithelium of Anodonta cygnea;Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology;2004-12

4. Extracellular potassium is necessary for acid translocation but not for acid formation in gastric mucosa;Biochimica et Biophysica Acta (BBA) - Molecular Cell Research;1990-08

5. A vacuolar type H(+)-ATPase regulates cytoplasmic pH in murine macrophages.;Journal of Biological Chemistry;1990-05

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