The role of myostatin and the calcineurin-signalling pathway in regulating muscle mass in response to exercise training in the rainbow troutOncorhynchus mykissWalbaum
Author:
Martin C. I.1, Johnston I. A.1
Affiliation:
1. Gatty Marine Laboratory, School of Biology, University of St Andrews,St Andrews, Scotland, KY16 8LB, UK
Abstract
SUMMARYRainbow trout Oncorhynchus mykiss Walbaum were exercised at 0.8 and 1.6 body lengths s-1 for 18 h a day over a 30 day period. Exercise resulted in a 24-30% increase in the average cross-sectional area of fast muscle fibres relative to tank-rested controls. The concentrations of growth factors and transcription factors hypothesised to play a role in regulating exercise-induced muscle fibre hypertrophy were measured. Exercise training resulted in a minor increase in calcineurin localisation in the nucleus. However, nuclear factor of T-cells 2 (NFAT2) nuclear localisation did not follow a pattern that was consistent with NFAT2-mediated transcriptional activity and changes in calcineurin signaling. The active peptide of myostatin, a negative regulator of muscle growth in mammals, was downregulated in exercise groups relative to tank-rested controls, but only by 6-7%. It was concluded that myostatin and calcineurin signaling do not play a major role in regulating exercise-induced muscle hypertrophy in trout.
Publisher
The Company of Biologists
Subject
Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics
Reference42 articles.
1. Amali, A. A., Lin, C. J.-F., Chen, Y.-H., Wang, W.-L., Gong,H.-Y., Lee, C.-Y., Ko, Y.-L., Lu, J.-K., Her, G. M., Chen, T. T. and Wu,J.-L. (2004). Upregulation of muscle-specific transcription factors during embryonic somitogenesis of zebrafish (Danio rerio) by knockdown of myostatin-1. Dev. Dyn.229,847-856. 2. Bass, J., Oldham, J., Sharma, M. and Kambadur, R.(1999). Growth factors controlling muscle development. Domest. Anim. Endocrinol.17,191-197. 3. Beals, C. R., Sheridan, C. M., Turck, C. W., Gardner, P. and Crabtree, G. R. (1997). Nuclear export of NF-ATc enhanced by glycogen synthase kinase-3. Science275,1930-1933. 4. Blough, E., Dinnen, B. and Esser, K. (1999). Extraction of nuclear proteins from striated muscle tissue. BioTechniques26,202-206. 5. Dolmetsch, R. E., Lewis, R. S., Goodnow, C. G. and Healy, J. I. (1997). Differential activation of transcription factors induced by Ca2+ response amplitude and duration. Nature386,855-858.
Cited by
47 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|