Inferring cell junction tension and pressure from cell geometry

Author:

Roffay Chloé12,Chan Chii J.3,Guirao Boris2,Hiiragi Takashi34,Graner François1ORCID

Affiliation:

1. Matière et Systèmes Complexes, Université de Paris - Diderot, CNRS UMR7057, 10 rue Alice Domon et Léonie Duquet, F-75205 Paris Cedex 13, France

2. Polarity, Division and Morphogenesis Team, Genetics and Developmental Biology Unit, (CNRS UMR3215/Inserm U934), Institut Curie, F-75248 Paris Cedex 05, France

3. European Molecular Biology Laboratory, 69117 Heidelberg, Germany

4. Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto 606-8501, Japan

Abstract

ABSTRACTRecognizing the crucial role of mechanical regulation and forces in tissue development and homeostasis has stirred a demand for in situ measurement of forces and stresses. Among emerging techniques, the use of cell geometry to infer cell junction tensions, cell pressures and tissue stress has gained popularity owing to the development of computational analyses. This approach is non-destructive and fast, and statistically validated based on comparisons with other techniques. However, its qualitative and quantitative limitations, in theory as well as in practice, should be examined with care. In this Primer, we summarize the underlying principles and assumptions behind stress inference, discuss its validity criteria and provide guidance to help beginners make the appropriate choice of its variants. We extend our discussion from two-dimensional stress inference to three dimensional, using the early mouse embryo as an example, and list a few possible extensions. We hope to make stress inference more accessible to the scientific community and trigger a broader interest in using this technique to study mechanics in development.

Funder

Marie Skłodowska-Curie

European Research Council

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

Reference88 articles.

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