Magnesium transport in freshwater teleosts

Abstract

The magnesium handling of freshwater teleost fish is discussed, with an emphasis on the role of branchial, intestinal and renal transport. In response to the eminent threat of constant diffusive losses of minerals such as magnesium, freshwater fish have developed efficient mechanisms for magnesium homeostasis. Magnesium losses are overcome by the uptake of magnesium from the food, making the intestine an important route for magnesium uptake. Some evidence suggests that intestinal magnesium uptake in fish is a regulated, cellular process. The ambient water is an additional magnesium source for fish, implicating the gills as a secondary route for magnesium uptake. Certainly, in some species, direct uptake from the water, probably via branchial routes, ameliorates the effects of a low-magnesium diet. The hard tissues, representing over 50 % of the total body magnesium pool, form a reservoir from which magnesium can be recruited to perform its functions in the cellular metabolism of soft tissues such as muscle. In fish, as in terrestrial vertebrates, the balance of a variety of elements becomes disturbed when the magnesium homeostasis of the soft tissues is disrupted. However, fish appear to be less sensitive than terrestrial vertebrates to these perturbations. Magnesium is reabsorbed in the kidneys to minimise losses. For renal cells, part of a cellular pathway has been elucidated that would allow absorptive magnesium transport (a magnesium conductive pathway in renal brush-border membranes). In some euryhaline teleosts, the kidneys appear to switch instantaneously to rapid magnesium secretion upon magnesium loading, a response common to marine fish that are threatened by diffusive magnesium entry. This enigmatic mechanism underlies the capacity of some euryhaline species to acclimate rapidly to sea water. Despite the progress made over the last decade, much of the cellular and molecular basis of magnesium transport in the gills, intestine and kidneys remains obscure. The application of fluorescent, radioactive and molecular probes, some of which have only recently become available, may yield rapid progress in the field of magnesium research.