Sec6 mutations and the Drosophila exocyst complex-Reference-Cited by-同舟云学术

Sec6 mutations and the Drosophila exocyst complex

Published:2005-03-15 Issue:6 Volume:118 Page:1139-1150
ISSN:1477-9137
Container-title:Journal of Cell Science
language:en
Short-container-title:

Author:

Murthy Mala¹,Ranjan Ravi¹,Denef Natalie²,Higashi Misao E. L.¹,Schupbach Trudi²,Schwarz Thomas L.¹

Affiliation:

1. Division of Neuroscience, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA

2. Howard Hughes Medical Institute, Molecular Biology Department, Princeton University, Princeton, NJ 08544, USA

Abstract

To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.

Publisher

The Company of Biologists

Subject

Cell Biology

Link

http://journals.biologists.com/jcs/article-pdf/118/6/1139/1521324/1139.pdf

Reference32 articles.

1. Adamo, J. E., Rossi, G. and Brennwald, P. (1999). The Rho GTPase Rho3 has a direct role in exocytosis that is distinct from its role in actin polarity. Mol. Biol. Cell10, 4121-4133.

2. Andres, A. J., Fletcher, J. C., Karim, F. D. and Thummel, C. S. (1993). Molecular analysis of the initiation of insect metamorphosis: a comparative study of Drosophila ecdysteroid-regulated transcription. Dev. Biol.160, 388-404.

3. Andrews, H. K., Zhang, Y. Q., Trotta, N. and Broadie, K. (2002). Drosophila Sec10 is required for hormone secretion but not general exocytosis or neurotransmission. Traffic3, 906-921.

4. Brymora, A., Valova, V. A., Larsen, M. R., Roufogalis, B. D. and Robinson, P. J. (2001). The brain exocyst complex interacts with RalA in a GTP-dependent manner: identification of a novel mammalian Sec3 gene and a second Sec15 gene. J. Biol. Chem.276, 29792-29797.

5. Finger, F. P. and Novick, P. (1997). Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol. Biol. Cell8, 647-662.

Cited by 57 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Exocyst stimulates multiple steps of exocytic SNARE complex assembly and vesicle fusion;Nature Structural & Molecular Biology;2024-09-06

2. The exocyst complex in neurological disorders;Human Genetics;2023-04-22

3. The exocyst complex is required for developmental and regenerative neurite growth in vivo;Developmental Biology;2022-12

4. The R-SNARE Ykt6 is required for multiple events during oogenesis in Drosophila;Cells & Development;2022-03

5. The C-terminal domain of SEC-10 is fundamental for exocyst function, Spitzenkörper organization and cell morphogenesis in Neurospora crassa;2022-01-14