Transcriptional regulation of epidermal cell fate in the Arabidopsis embryo

Abstract

How distinct cell fates are specified at correct positions within the plant embryo is unknown. In Arabidopsis, different cell fates are generated early on, starting with the two daughter cells of the zygote. To address mechanisms of position-dependent gene activation and cell fate specification,we analyzed the regulatory region of the Arabidopsis thaliana MERISTEM LAYER 1 (ATML1) gene, which is already expressed at the one-cell stage and whose expression is later restricted to the outermost, epidermal cell layer from its inception. A sensitive, multiple GFP reporter revealed a modular organization to the ATML1 promoter. Each region contributes positively to specific spatial and temporal aspects of the overall expression pattern, including position-dependent but auxin-independent regulation along the apical-basal axis of the embryo. A 101 bp fragment that conferred all aspects of ATML1 expression contained known binding sites for homeodomain transcription factors and other regulatory sequences. Our results suggest that expression patterns associated with cell fate determination in the plant embryo result from positional signals targeting different regulatory sequences in complex promoters.