Affiliation:
1. Department of Pharmacology, The University of Arizona College of Medicine, Tucson, AZ, USA
Abstract
Co-culture with glial cells and glia-conditioned media can induce blood-brain barrier properties in microvessel endothelial cells and protect against hypoxia-induced blood-brain barrier breakdown. We examined the effect of two types of glia-conditioned media on brain microvessel endothelial cell permeability and tight junction protein expression, and studied potential mechanisms of action. We found that C6-glioma-conditioned media, but not rat astrocyte-conditioned media, protected against an increase in permeability induced by exposure to 1% oxygen for 24 hours. This hypoxic stress caused an increase in the expression of tight junction proteins claudin-1 and actin,particularly in cells treated with C6-conditioned media. We found that C6-conditioned media has a significantly higher level of both basic fibroblast growth factor and vascular endothelial growth factor. Treatment with C6-conditioned media for 1 or 3 days protects against hypoxia-induced permeability increases, and this protective effect may be mediated by signal transduction pathways terminating at the transcription factor NFκB.
Publisher
The Company of Biologists
Reference49 articles.
1. Abbruscato, T. J., Thomas, S. A., Hruby, V. J. and Davis, T. P. (1997). Blood-brain barrier permeability and bioavailability of a highly potent and mu-selective opioid receptor antagonist, CTAP: comparison with morphine. J. Pharmacol. Exp. Ther.280, 402-409.
2. Abbruscato, T. J. and Davis, T. P. (1999). Combination of hypoxia/aglycemia compromises in vitro blood-brain barrier integrity. J. Pharmacol. Exp. Ther.289, 668-675.
3. Banks, W. A. (1999). Physiology and pathology of the blood-brain barrier: implications for microbial pathogenesis, drug delivery and neurodegenerative disorders. J. Neurovirol.5, 538-555.
4. Banks, W. A., Jaspan, J. B. and Kastin, A. J.(1997). Effect of diabetes mellitus on the permeability of the blood-brain barrier to insulin. Peptides18, 1577-1584.
5. Brouet, A., Sonveaux, P., Dessy, C., Balligand, J. L. and Feron,O. (2001). Hsp90 ensures the transition from the early Ca2+-dependent to the late phosphorylation-dependent activation of the endothelial nitric-oxide synthase in vascular endothelial growth factor-exposed endothelial cells. J. Biol. Chem.276, 32663-32669.
Cited by
90 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献