Affiliation:
1. Department of Immunology, Genetics and Pathology, Uppsala University, S-751 08, Uppsala, Sweden
Abstract
ABSTRACT
Fictive locomotion is frequently used to study locomotor output in paralyzed animals. We have evaluated the character of swim episodes elicited by different strategies in zebrafish. Motor output was measured on both sides of a body segment using electrodes and a pipeline for synchronizing stimulation and recording, denoising data and peak-finding was developed. The optomotor response generated swims most equivalent to spontaneous activity, while electrical stimulation and NMDA application caused various artefacts. Our optimal settings, optomotor stimulation using 5-day-old larvae, were combined with calcium imaging and optogenetics to validate the setup's utility. Expression of GCaMP5G by the mnx1 promoter allowed correlation of calcium traces of dozens of motor neurons to the fictive locomotor output. Activation of motor neurons through channelrhodopsin produced aberrant locomotor episodes. This strategy can be used to investigate novel neuronal populations in a high-throughput manner to reveal their role in shaping motor output.
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Funder
the Kjell and Märta Beijers Foundation
Jeanssons foundation
Carl Tryggers Foundation
Brain Foundation
Swedish Research Council
Magnus Bergvalls Foundation
Royal Swedish Academy of Sciences
Max and Edit Follins Foundation
Åke Wibergs Foundation
Olle Engkvist stiftelse
Ragnar Söderberg Foundation
Swedish foundation for strategic research
Uppsala University: Uppsala Universitet
Publisher
The Company of Biologists
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology
Cited by
4 articles.
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