Affiliation:
1. Centro de Biología Molecular Severo Ochoa, CSIC-UAM, c/ Nicolás Cabrera 1, Madrid 28049, Spain
2. CIBER de Enfermedades Raras (CIBERER), c/ Nicolás Cabrera 1, Madrid 28049, Spain
Abstract
Placodes are discrete thickenings of the vertebrate cranial ectoderm that generate morpho-functionally distinct structures, such as the adenohypophysis, olfactory epithelium and lens. All placodes arise from a horseshoe-shaped preplacodal ectoderm in which the precursors of individual placodes are intermingled. However, fate-map studies indicated that cells positioned at the preplacodal midline give rise to only the adenohypophyseal placode, suggesting a unique organization of these precursors within the preplacode. To test this possibility, we combined embryological and molecular approaches in chick embryos to show that, at gastrula stage, adenohypophyseal precursors are clustered in the median preplacodal ectoderm, largely segregated from those of the adjacent olfactory placode. Median precursors are elongated, densely packed and, at neurula stage, express a molecular signature that distinguishes them from the remaining preplacodal cells. Olfactory placode precursors and midline neural cells can replace ablated adenohypophyseal precursors up to head-fold stage, although with a more plastic organization. We thus propose that adenohypophyseal placode precursors are unique within the preplacodal ectoderm possibly because they originate the only single placode and the only one with an endocrine character.
Funder
Secretaría de Estado de Investigación, Desarrollo e Innovació
Comunidad Autonoma de Madrid
Centro de Investigación Biomédica en Red de Enfermedades Raras
Instituto de Salud Carlos III
Fundacion Ramon Areces
Consejo Superior de Investigaciones Científicas
JAEDOC-012
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献