Inhibition of PrPSc formation by lentiviral gene transfer of PrP containing dominant negative mutations-Reference-Cited by-同舟云学术

Inhibition of PrPSc formation by lentiviral gene transfer of PrP containing dominant negative mutations

Published:2004-11-01 Issue:23 Volume:117 Page:5591-5597
ISSN:1477-9137
Container-title:Journal of Cell Science
language:en
Short-container-title:

Author:

Crozet Carole¹,Lin Yea-Lih²,Mettling Clément²,Mourton-Gilles Chantal³,Corbeau Pierre²,Lehmann Sylvain¹⁴,Perrier Véronique¹

Affiliation:

1. Laboratoire de Biologie des Encéphalopathies Spongiformes, CNRS UPR 1142, 141 rue de la Cardonille, 34396 Montpellier CEDEX 5, France

2. Laboratoire Lentivirus et Transfert de Gènes – Institut de Génétique Humaine, CNRS UPR 1142, 141 rue de la Cardonille, 34396 Montpellier CEDEX 5, France

3. BIO-RAD, Centre de Pharmacologie et Biotechnologies pour la Santé, CNRS UMR 5094, 15 avenue C. Flahault, 34060 Montpellier CEDEX 2, France

4. Laboratoire de Biochimie. Hopital St Eloi, 80 avenue A. Fliche 34295 Montpellier CEDEX 5, France

Abstract

Currently, there is no treatment to cure transmissible spongiform encephalopathies. By taking advantage of the `prion-resistant' polymorphisms Q171R and E219K that naturally exist in sheep and humans, respectively, we have evaluated a therapeutic approach of lentiviral gene transfer. Here, we show that VSV-G (vesicular stomatitis virus G glycoprotein) pseudotyped FIV-(feline immunodeficiency virus) derived vectors carrying the mouse Prnp gene in which these mutations have been inserted, are able to inhibit prion replication in chronically prion-infected cells. Because lentiviral tools are able to transduce post-mitotic cells such as neurons or cells of the lymphoreticular system, this result might help the development of gene- or cell-therapy approaches to prion disease.

Publisher

The Company of Biologists

Subject

Cell Biology

Link

http://journals.biologists.com/jcs/article-pdf/117/23/5591/1530780/5591.pdf

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