Affiliation:
1. Cell Biology Group, University of Bielefeld, POB 8640, D-4800 Bielefeld, FRG
Abstract
Summary
Microinjection of specific antibodies can be an alternative and a supplement to genetic engineering in dissecting the function of individual cytoskeletal components. In this report, we describe some of the requirements for using this technique, its potential application in conjunction with morphological and biochemical analyses, and its limitations. Examples are given for the injection of antibodies to α-actinin, vinculin and myosin, and the effects of such treatment on adhesion, motility and cytokinesis of the recipient cells.
Publisher
The Company of Biologists
Cited by
9 articles.
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