The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics

Author:

Li Yang1,Merkel Chelsea D.1,Zeng Xuemei2,Heier Jonathon A.1,Cantrell Pamela S.2,Sun Mai2,Stolz Donna B.1,Watkins Simon C.1,Yates Nathan A.123,Kwiatkowski Adam V.1ORCID

Affiliation:

1. Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA

2. Biomedical Mass Spectrometry Center, University of Pittsburgh Schools of the Health Sciences, Pittsburgh, PA 15261, USA

3. University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, PA 15232, USA

Abstract

The junctional complexes that couple cardiomyocytes must transmit the mechanical forces of contraction while maintaining adhesive homeostasis. The adherens junction (AJ) connects the actomyosin networks of neighboring cardiomyocytes and is required for proper heart function. Yet little is known about the molecular composition of the cardiomyocyte AJ or how it is organized to function under mechanical load. Here we define the architecture, dynamics and proteome of the cardiomyocyte AJ. Mouse neonatal cardiomyocytes assemble stable AJs along intercellular contacts with organizational and structural hallmarks similar to mature contacts. We combine quantitative mass spectrometry with proximity labeling to identify the N-cadherin (CDH2) interactome. We define over 350 proteins in this interactome, nearly 200 of which are unique to CDH2 and not part of the E-cadherin (CDH1) interactome. CDH2-specific interactors are comprised primarily of adaptor and adhesion proteins that promote junction specialization. Our results provide novel insight into the cardiomyocyte AJ and provide a proteomic atlas for defining the molecular complexes that regulate cardiomyocyte intercellular adhesion.

Funder

National Institutes of Health

National Cancer Institute

Publisher

The Company of Biologists

Subject

Cell Biology

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