Sequence environment of BMP-dependent activating elements controls transcriptional responses to Dpp signaling in Drosophila

Abstract

Intercellular signaling pathways activate transcription factors, which along with tissue-specific co-factors regulate expression of target genes. Responses to TGF-ß/BMP signals are mediated by Smad proteins, which form complexes and accumulate in the nucleus to directly bind and regulate enhancers of BMP-targets upon signaling. In Drosophila, gene activation by BMP signaling often requires, in addition to direct input by Smads, the signal-dependent removal of the transcriptional repressor Brk. Previous studies on enhancers of BMP activated genes have defined a BMP responsive motif, the AE, which integrates activatory and repressive input by the Smad complex and Brk, respectively. Here, we address whether sequence variations within the core AE sequences might endow the motif with additional properties accounting for qualitative and quantitative differences in BMP responses including tissue-specificity of transcriptional activation and differential sensitivity to Smad and Brk inputs. By analyzing and cross-comparing three distinct BMP responsive enhancers from the genes wit and dad in two different epithelia, the wing imaginal disc and the follicular epithelium, we demonstrate that differences in the AEs neither contribute to the observed tissue-restriction of BMP responses nor to differences in the utilization of the Smad and Brk branches for transcriptional activation. Our results rather suggest that the cis-environment of the BMP response elements not only dictate tissue specificity but also differential sensitivity to the two BMP mediators.