Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome-Reference-Cited by-同舟云学术

Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome

Published:2003-09-01 Issue:17 Volume:116 Page:3623-3634
ISSN:1477-9137
Container-title:Journal of Cell Science
language:en
Short-container-title:

Author:

Eckert Jörg H.¹,Johnsson Nils²

Affiliation:

1. Ruhr-Universität Bochum, Institut für Physiologische Chemie,Medizinische Fakultät, 44780 Bochum, Germany

2. Institut für Toxikologie und Genetik, Forschungszentrum Karlsruhe,Postfach 3640, 76021 Karlsruhe, Germany

Abstract

The protein import machinery of the peroxisome consists of many proteins,collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub-and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisiae. We found that Pex10p plays a central role in the protein interaction network by connecting the ubiquitin conjugation enzyme Pex4p to the other members of the protein import machinery. A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions.

Publisher

The Company of Biologists

Subject

Cell Biology

Link

http://journals.biologists.com/jcs/article-pdf/116/17/3623/1362267/3623.pdf

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